| Primary Identifier | MGI:6108112 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Hltf |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The genomic sequence for the coding part of exon 1 plus exons 2-5 (coding for amino-acids 2-208) was replaced with a lacZ reporter gene cassette and a loxP site flanked neomycin resistance gene cassette. The neo cassette was removed through subsequent Cre-mediated recombination, leaving a combined reporter null allele. Northern blot and RT-PCR analysis confirmed the absence of endogenous transcripts from this allele. |
