| Primary Identifier | MGI:6117673 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Oprm1 |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 4 and a lox2272 site was inserted downstream of exon 4 to allow for conditional removal of the wild-type exon 4 and expression of a mutagenized cDNA for exons 4 and 5 with nucleotide substitutions that result in the amino acid substitutions of alanine for 11 serine or threonine. An FRT-flanked neomycin resistance cassette was inserted downstream of the lox2272 site in intron 4. The selection cassette was removed. |
