| Primary Identifier | MGI:6111193 | Allele Type | Endonuclease-mediated |
| Attribute String | Inserted expressed sequence | Gene | Tcte1 |
| Transmission | Germline | Strain of Origin | (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Using an sgRNA and CRISPR/Cas9 technology, a FLAG sequence was inserted at the 3' end of the coding sequence immediately upstream of the stop codon. The sgRNA- and Cas9-expressing plasmid and reference plasmid were transfected into EGR-G01 ES cells. The ES cells were then injected into 8-cell ICR embryos. |
