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Allele : Tcte1<em1Osb> t-complex-associated testis expressed 1; endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
Primary Identifier  MGI:6111193 Allele Type  Endonuclease-mediated
Attribute String  Inserted expressed sequence Gene  Tcte1
Transmission  Germline Strain of Origin  (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb
Is Recombinase  false Is Wild Type  false
molecularNote  Using an sgRNA and CRISPR/Cas9 technology, a FLAG sequence was inserted at the 3' end of the coding sequence immediately upstream of the stop codon. The sgRNA- and Cas9-expressing plasmid and reference plasmid were transfected into EGR-G01 ES cells. The ES cells were then injected into 8-cell ICR embryos.
  • mutations:
  • Insertion
  • synonyms:
  • Tcte1<em1Maik>,
  • Tcte1<FLAG1>,
  • Tcte1<tm2(Maik)>,
  • Tcte1<FLAG1>,
  • Tcte1<em1Maik>,
  • Tcte1<tm2(Maik)>
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1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

Trail: Allele

0 Driven By

4 Publication categories

Trail: Allele




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