| Primary Identifier | MGI:6140227 | Allele Type | Targeted |
| Attribute String | Modified isoform(s), No functional change | Gene | Iftap |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A self-excising cassette, containing a tACE promoter, a cre gene, HSV-TK poly(A) sequences and a neomycin resistance gene cassette, was used to target the promoter. This resulted in a 644 bp deletion encompassing the promoter and exon 1. The deleted sequence is also part of intron 2 of the Rag2 gene. RT-PCR experiments show that Rag2 is not affected, but that the targeted locus shows transcription from an alternative promoter with an alternative first exon 1b. |
