| First Author | Murdoch B | Year | 2010 |
| Journal | Mamm Genome | Volume | 21 |
| Issue | 11-12 | Pages | 550-5 |
| PubMed ID | 21113599 | Mgi Jnum | J:166751 |
| Mgi Id | MGI:4849570 | Doi | 10.1007/s00335-010-9303-5 |
| Citation | Murdoch B, et al. (2010) Multiple loci contribute to genome-wide recombination levels in male mice. Mamm Genome 21(11-12):550-5 |
| abstractText | Recent linkage-based studies in humans suggest the presence of loci that affect either genome-wide recombination rates, utilization of recombination hotspots, or both. We have been interested in utilizing cytological methodology to directly assess recombination in mammalian meiocytes and to identify recombination-associated loci. In the present report we summarize studies in which we combined a cytological assay of recombination in mouse pachytene spermatocytes with QTL analyses to identify loci that contribute to genome-wide levels of recombination in male meiosis. Specifically, we analyzed MLH1 foci, a marker of crossovers, in 194 F2 male mice derived from a subspecific cross between CAST/EiJ and C57BL/6J parental strains. We then used these data to uncover loci associated with individual variation in mean MLH1 values. We identified seven recombination-associated loci across the genome (on chromosomes 2, 3, 4, 14, 15, 17, and X), indicating that there are multiple recombination 'setting' loci in mammalian male meiosis. |
