| Primary Identifier | MGI:6115603 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Depdc5 |
| Strain of Origin | C57BL/6N | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 mediated mutagenesis created a 22 base pair deletion (c77_99del/L23fsX65) resulting in a frameshift in exon 2. CRISPR guides target 5'-TGATGTGAGGAAATACTTT-3' and 5'-GTATTTCCTCACATCAAACT-3'. No difference in transcript level compared to wild-type is seen, however mass spectrometry of parallel reaction monitoring confirmed absence of DEPDC5 peptides indicating a null allele. |
