| Primary Identifier | MGI:6147689 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Spata16 |
| Transmission | Germline | Strain of Origin | (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb |
| Is Recombinase | false | Is Wild Type | false |
| description | EGR-G01 cells carry one copy of Tg(CAG-EGFP,Acr-EGFP)2Osb. |
| molecularNote | A point mutation (G to A) was introduced in codon 851 of exon 4, predicted to result in a R284Q alteration in the encoded protein. This mouse mutation corresponds to the single amino acid substitution (c.848G>A or R283Q) identified in infertile globozoospermic patients. RT-PCR analysis indicated that the point mutation caused no splicing abnormality. |
