| Primary Identifier | MGI:6147690 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Spata16 |
| Transmission | Germline | Strain of Origin | (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb |
| Is Recombinase | false | Is Wild Type | false |
| description | EGR-G01 cells carry one copy of Tg(CAG-EGFP,Acr-EGFP)2Osb. |
| molecularNote | CRISPR mutagenesis was used to generate a 781 bp deletion that removed 88 bp out of 90 bp of exon 4 which encodes the C-terminus of the tetratricopeptide repeat (TPR) domain. This deletion resulted in the mistranslation of the remaining 2 bp of the mutant fourth exon. |