Primary Identifier | MGI:6148035 | Allele Type | Targeted |
Attribute String | Epitope tag, Inserted expressed sequence, Null/knockout | Gene | Map2k1 |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A cDNA of Map2k1 (tagged with Myc and flanked by loxP sites), an frt-flanked neomycin selection cassette, and a cDNA of Map2k2 were inserted into the first exon of Mek1. Western blot analysis showed that both endogenous Map2k1 protein and the Myc-tagged Map2k1 protein are expressed in heterozygous embryonic stem (ES) cells. |