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Allele : Ano5<tm1Lrk> anoctamin 5; targeted mutation 1, Louise R Rodino-Klapac
Primary Identifier  MGI:6149153 Allele Type  Targeted
Attribute String  Conditional ready, Null/knockout, Reporter Gene  Ano5
Transmission  Germline Strain of Origin  Not Specified
Is Recombinase  false Is Wild Type  false
description  Vector derived from PG00097_Z_1_G0 KOMP cell line.
molecularNote  A knockout-first allele was created using the Ano5 vector. The following elements were inserted into the locus: an FRT site, an exogenous intron, an IRES, the lacZ reporter gene, SV40 polyA features, a loxP site, a neomycin resistance gene cassette, a second FRT site and a second loxP site into intron 8. A third loxP site was inserted into intron 9. RT-PCR confirmed the loss of transcription from exon 9 onwards. qRT-PCR showed overall reduction of 80% in transcription from this allele. Western blots confirmed the lack of protein expression from this allele.
  • mutations:
  • Insertion
  • synonyms:
  • Ano5<->,
  • Ano5<->
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

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0 Driven By

6 Publication categories





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