| First Author | Demaria MC | Year | 2020 |
| Journal | iScience | Volume | 23 |
| Issue | 5 | Pages | 101104 |
| PubMed ID | 32428859 | Mgi Jnum | J:338660 |
| Mgi Id | MGI:6718186 | Doi | 10.1016/j.isci.2020.101104 |
| Citation | Demaria MC, et al. (2020) Tetraspanin CD53 Promotes Lymphocyte Recirculation by Stabilizing L-Selectin Surface Expression. iScience 23(5):101104 |
| abstractText | Tetraspanins regulate key processes in immune cells; however, the function of the leukocyte-restricted tetraspanin CD53 is unknown. Here we show that CD53 is essential for lymphocyte recirculation. Lymph nodes of Cd53(-/-) mice were smaller than those of wild-type mice due to a marked reduction in B cells and a 50% decrease in T cells. This reduced cellularity reflected an inability of Cd53(-/-) B and T cells to efficiently home to lymph nodes, due to the near absence of L-selectin from Cd53(-/-) B cells and reduced stability of L-selectin on Cd53(-/-) T cells. Further analyses, including on human lymphocytes, showed that CD53 stabilizes L-selectin surface expression and may restrain L-selectin shedding via both ADAM17-dependent and ADAM17-independent mechanisms. The disruption in lymphocyte recirculation in Cd53(-/-) mice led to impaired immune responses dependent on antigen delivery to lymph nodes. Together these findings demonstrate an essential role for CD53 in lymphocyte trafficking and immunity. |
