| Primary Identifier | MGI:6152666 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Cabyr |
| Strain of Origin | (C57BL/6NJcl x DBA/2NJcl)F2 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Exon 2, which contains the ATG translation start codon and is shared amongst all known splice variants, was targeted using an sgRNA and CRISPR/Cas9. The resulting allele has a 17 bp insertion in exon 2, resulting in a reading frame shift and subsequent premature stop codon in exon 3. Immunoblots of extracts from spermatozoa demonstrate the lack of protein expression from this allele. |
