| Primary Identifier | MGI:6153339 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Far1 |
| Strain of Origin | C57BL/6N | Is Recombinase | false |
| Is Wild Type | false | Project Collection | IMPC |
| molecularNote | This allele from IMPC was generated at the Welcome Trust Sanger Institute. Four sgRNAs were used to target the 5â² and 3â² intronic flanking regions thereby deleting in frame exon 5, which encodes for 50 amino acids. Sanger Sequencing confirmed the CRISPR/Cas9-mediated deletion of 475 bp including the deletion of the entire exon 5. Western blot analysis demonstrated absence of protein expression in testis extracts from homozygous mutant male mice. |
