| First Author | Zhou Y | Year | 2014 |
| Journal | Biochem Biophys Res Commun | Volume | 446 |
| Issue | 4 | Pages | 959-64 |
| PubMed ID | 24642257 | Mgi Jnum | J:219123 |
| Mgi Id | MGI:5619682 | Doi | 10.1016/j.bbrc.2014.03.033 |
| Citation | Zhou Y, et al. (2014) The formation of brown adipose tissue induced by transgenic over-expression of PPARgamma2. Biochem Biophys Res Commun 446(4):959-64 |
| abstractText | Brown adipose tissue (BAT) is specialized to dissipate energy as heat, therefore reducing fat deposition and counteracting obesity. Brown adipocytes arise from myoblastic progenitors during embryonic development by the action of transcription regulator PRDM16 binding to PPARgamma, which promotes BAT-like phenotype in white adipose tissue. To investigate the capability of converting white adipose tissue to BAT or browning by PPARgamma in vivo, we generated transgenic mice with over-expressed PPARgamma2. The transgenic mice showed strong brown fat features in subcutaneous fat in morphology and histology. To provide molecular evidences on browning characteristics of the adipose tissue, we employed quantitative real-time PCR to determine BAT-specific gene expressions. The transgenic mice had remarkably elevated mRNA level of UCP1, Elovl3, PGC1alpha and Cebpalpha in subcutaneous fat. Compared with wild-type mice, UCP1 protein levels were increased significantly in transgenic mice. ATP concentration was slightly decreased in the subcutaneous fat of transgenic mice. Western blotting analysis also confirmed that phosphorylated AMPK and ACC proteins were significantly (P<0.01) increased in the transgenic mice. Therefore, this study demonstrated that over-expression of PPARgamma2 in skeletal muscle can promote conversion of subcutaneous fat to brown fat formation, which can have beneficial effects on increasing energy metabolisms and combating obesity. |
