| Primary Identifier | MGI:6162684 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Pm20d1 |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 targeting is used to introduce an out-of-frame deletion of 6 basepairs in exon 1 that results in the conversion of a phenylalanine codon into a premature stop codon (TTC-->TAG). Sanger sequencing confirmed the presence of a new T-A junction and a new stop codon in exon 1 at the 43rd amino acid position. qPCR analysis confirmed the modification was present in its mRNA. Shotgun proteomics confirmed selective loss of PM20D1 protein from liver. |
