| Primary Identifier | MGI:6154949 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Shisa7 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted into intron 1 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 3. The neo cassette was removed through subsequent flp-mediated recombination and exons 2 and 3 were deleted through subsequent cre-mediated recombination. Exon 2 contains the ATG translation start site. Immunoblots of hippocampal crude synaptic membrane fractions confirmed the lack of protein expression from this allele. |
