| Primary Identifier | MGI:6156567 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Stat2 |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector was created using mouse Stat2 sequences from C57BL/6J BAC RPCIB-731 and human STAT2 sequences from human BAC RPCIB-753. The targeting vector also inserted an FRT-flanked neomycin resistance cassette in intron 1 and a F3-flanked puromycin resistance cassette in intron 24. The vector is designed replace the endogenous mouse Stat2 coding sequence (spanning from part of exon 2 through part of exon 24) with the corresponding human STAT2 coding sequence. The allele retains the endogenous mouse Stat2 5' untranslated region, promoter sequences (exon 1 and part of exon 2) and 3' untranslated region. Flp-mediated recombination removed the FRT-flanked neo and puro cassettes. |
