| Primary Identifier | MGI:6157366 | Allele Type | Targeted |
| Attribute String | Conditional ready, Null/knockout, Reporter | Gene | Bsph2 |
| Transmission | Germline | Strain of Origin | (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | This targeted knockout-first reporter allele with conditional potential (conditional-ready) was generated by electroporating ES cells with the EUCOMM vector PG00143_Z_7_G04 to insert a DNA sequence containing a FRT site; a lacZ cassette containing the En2 splice acceptor and a polyadenylation signal (p[A]); a loxP site; a promoter-driven neomycin resistance cassette with a p[A]; a second FRT site; and a second loxP site into the second intron and a third loxP site into the fourth intron of the mouse gene. |
