| Primary Identifier | MGI:6160101 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Cdk1 |
| Transmission | Germline | Strain of Origin | 129S1/SvImJ or C57BL/6J |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A splice acceptor-STOP/neomycin selection cassette flanked by loxP sites was introduced on the 5 end of exon 3, which was modified to introduce point mutations that generate a threonine to alanine substitution at amino acid 14 (T14A) and a tyrosine to phenylalanine substitution at amino acid 15 (Y15F). A14 and F15 cannot be phosphorylated and thus this mutant becomes non-inhibitable by phosphorylation. Cre-mediated recombination is necessary to remove the loxP-flanked STOP cassette and get expression of the mutant protein. |
