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Allele : Gt(ROSA)26Sor<tm1.1(CAG-tdTomato/GCaMP6f)Mdcah> gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Michael Cahalan
Primary Identifier  MGI:6157515 Allele Type  Targeted
Attribute String  Conditional ready, Epitope tag, Reporter Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  C57BL/6N
Is Recombinase  false Is Wild Type  false
molecularNote  The targeting vector contains (5' to 3'): CAG (cytomegalovirus early enhancer/chicken beta-actin) promoter, an FRT site, loxP-STOP-loxP cassette, Salsa6f (see below), WPRE, bGHpA, AttB site, an FRT site, neomycin selection cassette, AttP site (using the Addgene Plasmid #34883); inserted into the Gt(ROSA)26Sor locus. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence. Salsa6f contains tdTomato linked to GCaMP6f by a V5 epitope tag.
  • mutations:
  • Insertion
  • synonyms:
  • Gt(ROSA)26Sor<pCAG-FRT-LSL-Salsa6f-WPRE-bGHpA-AttB/P>,
  • LSL-Salsa6f,
  • Gt(ROSA)26Sor<pCAG-FRT-LSL-Salsa6f-WPRE-bGHpA-AttB/P>,
  • LSL-Salsa6f
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

0 Driven By

12 Publication categories





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