Primary Identifier | MGI:6157515 | Allele Type | Targeted |
Attribute String | Conditional ready, Epitope tag, Reporter | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | C57BL/6N |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting vector contains (5' to 3'): CAG (cytomegalovirus early enhancer/chicken beta-actin) promoter, an FRT site, loxP-STOP-loxP cassette, Salsa6f (see below), WPRE, bGHpA, AttB site, an FRT site, neomycin selection cassette, AttP site (using the Addgene Plasmid #34883); inserted into the Gt(ROSA)26Sor locus. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence. Salsa6f contains tdTomato linked to GCaMP6f by a V5 epitope tag. |