| Primary Identifier | MGI:6164038 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Thsd4 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CTACCTTGATTCTGCAGAAT and ACTGGACCCATGTGGACACA, which resulted in a 516 bp deletion beginning at Chromosome 9 position 60,428,091 bp and ending after 60,428,606 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000636311 (exon 4) and 154 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 34 and early truncation 52 amino acids later. |