| First Author | Renzel R | Year | 2013 |
| Journal | Glia | Volume | 61 |
| Issue | 7 | Pages | 1146-54 |
| PubMed ID | 23633386 | Mgi Jnum | J:358713 |
| Mgi Id | MGI:6855062 | Doi | 10.1002/glia.22505 |
| Citation | Renzel R, et al. (2013) Polarized distribution of AMPA, but not GABAA , receptors in radial glia-like cells of the adult dentate gyrus. Glia 61(7):1146-54 |
| abstractText | Glial fibrillary acidic protein (GFAP)-positive astrocytes with radial processes [radial glia (RG)-like cells] in the postnatal dentate gyrus share many of the characteristics of embryonic radial glia and appear to act as precursor cells for adult dentate neurogenesis, a process important for pattern separation and hippocampus-dependent learning. Although much work has delineated the mechanisms underlying activity-neurogenesis coupling via gamma-amino butyric acid (GABA)ergic neurotransmission on GFAP-negative transient-amplifying cells and neuroblasts, little is known regarding the effects of neurotransmitters on RG-like cells. Conflicting evidence exists for both GABA and glutamate receptors on these cells. Here, using GFAP reporter mice, we show that the somatic membrane of RG-like cells carries GABAA receptors and glutamate transporters but not ionotropic glutamate receptors, whereas 2-amino-3-(hydroxyl-5-methylisoxazole-4-yl) propionic acid (AMPA) and GABAA receptors are expressed on the processes of these cells. Almost all RG-like cells expressed the GluA2 subunit, which restricts the Ca(2+) permeability of AMPA receptors. The glial GABAA receptors mainly comprised alpha2/alpha4, beta1, and gamma1/gamma3. The selective presence of AMPA receptors on the radial processes may be important for sensing and responding to local activity-driven glutamate release and supports the concept that RG-like astrocytes are composed of functional and structural domains. |
