| Primary Identifier | MGI:6188987 | Allele Type | Endonuclease-mediated |
| Attribute String | Conditional ready | Gene | Tbc1d14 |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Exon 3 of the gene was targeted for flanking insertions of loxP sites. lssDNA (long single-stranded DNA) containing sequence for a loxP site, the end of intron 2, the entire exon 3, the start of intron 3 and a second loxP site was microinjected into zygotes together with two sgRNAs and Cas9 mRNA (with 81 bp poly(A) tail appended). This conditional-ready allele was found in founders 1, 2, 3 and 4. |
