Primary Identifier | MGI:6191749 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Klhl14 |
Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The L1L2_Bact_P cassette was inserted at position 21757262 of Chromosome 18 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 21759352. Cre-mediated recombination removed exon 3 and the selection cassette leaving behind the reporter cassette. |