| Primary Identifier | MGI:6196512 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Rubcn |
| Strain of Origin | (C57BL/6N x C57BL/6J)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Aspartic acid codon 178 in exon 5 was changed to premature stop codon TAA using an sgRNA and a 183 nt single-stranded DNA oligonucleotide template with CRISPR/Cas9 technology. Two additional substitutions were introduced immediately downstream to create an EcoRI restriction site for diagnostic purposes. The mutation is encoded NM_001200038.1:c.562_569delinsTAAGAAT. All necessary components were microinjected into the cytoplasm of C57BL/6 zygotes. |
