Primary Identifier | MGI:6202418 | Allele Type | Targeted |
Attribute String | Conditional ready, Reporter | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | C57BL/6JJic |
Is Recombinase | false | Is Wild Type | false |
description | ES cell line = Wit 9 (C57BL/6JJic). |
molecularNote | A construct containing the CAG promoter with a VloxP-STOP-VloxP cassette preceding EGFP sequence was inserted into the intron of the Gt(ROSA)26Sor locus via homologous recombination in Wit 9 ES cells. VCre-mediated recombination of this allele results in the expression of the EGFP reporter driven by the CAG promoter. VCre/VloxP is a site-specific recombination system, which was derived from plasmid p0908 of Vibrio sp. VCre is a recombinase that recognizes its own specific target sites (VloxP). |