| Primary Identifier | MGI:6196354 | Allele Type | Targeted |
| Attribute String | Null/knockout, RMCE-ready | Gene | Rbp4 |
| Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A null allele was created by replacing the ATG start site within exon 2 with a PGK-neo cassette flanked by lox71 and loxP sites. Besides disrupting the gene, the cassette also serves as an acceptor in recombination mediated cassette exchange (RMCE). RT-PCR confirmed absence of mRNA in the brain, lung, liver, and kidney. Northern blots of liver RNA and Western blots of serum confirmed absence of mRNA and protein, respectively. |
