| Primary Identifier | MGI:6202624 | Allele Type | Targeted |
| Attribute String | Not Specified | Gene | Siglecg |
| Transmission | Germline | Strain of Origin | BALB/cJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An arginine to glutamic acid (R120E) mutation was inserted in exon 3 and a floxed neo cassette was inserted in intron 4 via homologous recombination. Cre-mediated recombination removed the neo cassette. The exon 3 mutation changes a conserved positively charged amino acid in the first ligand binding domain to a negatively charged amino acid. The mutant protein shows surface expression in B-cells similar to wild-type protein but fails to bind ligand. |
