| Primary Identifier | MGI:6197715 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence | Gene | Trem2 |
| Strain of Origin | (C57B/6J x DBA/2J)F1 x FVB/N | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The allele was generated by injecting cas9 nuclease and a single guide RNA designed to introduce an arginine to histidine (R27H) point mutation with three silent mutations GA>TC to create a restriction enzyme site for genotyping and G>A to block the protospacer-adjacent motif to allow higher editing efficiency. Western blot of microglia and bone marrow derived macrophages confirms a reduction in protein levels. |
