| Primary Identifier | MGI:6195289 | Allele Type | Targeted |
| Attribute String | Epitope tag, Reporter | Gene | Arc |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Sequence coding for a TAP (Tandem Affinity Purification) tag (containing Histidine Affinity Tag (HAT), TEV protease and 3x Flag sequences), a linker (coding for GGGS), and the Venus yellow fluorescent reporter, followed by a loxP site flanked neomycin resistance gene cassette, was inserted before the translation stop codon in exon 1. The neo cassette was removed through subsequent cre-mediated recombination. This leaves an allele coding for a chimeric protein of the endogenous protein with a C-terminal TAP tag and Venus peptide. |
