| Primary Identifier | MGI:6197919 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Vps35 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 86009499 of Chromosome 8 upstream of exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 86011277. The critical exon is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression. Subsequent cre expression results in a knockout allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |
