| Primary Identifier | MGI:6198591 | Allele Type | Targeted |
| Attribute String | Inducible, Null/knockout, Recombinase | Gene | Ly6a |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | A cDNA encoding a MerCreMer fusion protein and an FRT-flanked neomycin cassette were recombined in-frame with the Ly6a ATG start codon (exon 2) via homologous recombination in embryonic stem (ES) cells. The MerCreMer double fusion protein incorporates a cre recombinase cDNA sequence flanked by mutated murine estrogen receptor (mer) ligand binding domains (amino acids 281-599, G525R). Resultant mice were crossed with Flpe mice to delete the neomycin cassette. Expression of Ly6a is eliminated in the targeted allele. |
