| Primary Identifier | MGI:6203633 | Allele Type | Targeted |
| Attribute String | Inducible, Null/knockout, Recombinase | Gene | Ager |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | The targeting vector was designed to insert a cre/ERT2 fusion gene, followed by a polyadenylation signal and a frt-flanked neomycin resistance cassette, into the start codon of Ager gene. The construct was electroporated into ES cells for homologous recombination. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred with FLP-deleter mice to remove the neo cassette. It is a null allele. Ager protein is not detected in lungs of the homozygous mice. |
