| Primary Identifier | MGI:6258721 | Allele Type | Targeted |
| Attribute String | Conditional ready, Null/knockout | Gene | Usp29 |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site and a neomycin resistance gene cassette (in the same transcriptional direction as the targeted gene) were inserted into intron 1. A second loxP site was inserted into intron 1 of upstream neighbor Peg3 (with which it shares a bidirectional promoter) upstream of the Peg3-DMR (differentially methylated region). RT-PCR experiments showed that the insertion of the neo cassette, which contains a poly(A) signal, dramatically reduces the expression of the targeted gene. |
