| Primary Identifier | MGI:6275151 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Spink5 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences GCATCCATGAAAGATCATCA and TTCCCAATGCTCCAGAGACG, which resulted in a 412 bp deletion beginning at Chromosome 18 position 43,966,767 bp and ending after 43,967,178 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000438072 (exon 3) and 284 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 27 and early truncation 21 amino acids later. |
