| Primary Identifier | MGI:6280025 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Hmgcs1 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TCTACACTGCAAAGAGAGCA and ACTTACTACCTCTCTAGAAC, which resulted in a 548 bp deletion beginning at Chromosome 13 position 119,702,388 bp and ending after 119,702,935 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001063943 (exon 5) and 382 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 246 and early truncation 4 amino acids later. |
