| Primary Identifier | MGI:6280383 | Allele Type | Endonuclease-mediated |
| Attribute String | Conditional ready | Gene | Ptcd1 |
| Strain of Origin | C57BL/6N | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology introduced loxP sites flanking exon 3 and also moved two bases from the 5' end of exon 4 to the 3' end of exon 3 to enable a frameshift upon conditional deletion of exon 3. Sequencing confirmed that the engineered exon-3 and exon-4 junctions are canonically spliced to produce an mRNA that is identical to the mRNA from wild-type mice. mRNA levels are comparable between homozygotes and wild-type mice. |
