| Primary Identifier | MGI:6273151 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Mroh2b |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences AGTGGTTTCCTTCAGACCCA and GCTTTAATAGCTTAAAGTAT, which resulted in a 1100 bp deletion beginning at Chromosome 15 position 4,904,805 bp and ending after 4,905,904 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000124014 and ENSMUSE00000124016 (exons 5 and 6) and 846 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 116 and early truncation 5 amino acids later. |
