| Primary Identifier | MGI:6272660 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Usp35 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TGTTACTTATATCTATCCCA and CCAGCATGAACTCTTCCGAA, which resulted in a 429 bp deletion beginning at Chromosome 7 position 97,324,102 bp and ending after 97,324,530 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000906983 (exon 2) and 296 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 224 and early truncation 1 amino acid later. |
