| Primary Identifier | MGI:6277993 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Iws1 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences AGCATGCTTCCAGATGTGGA and AGTAAAAACTGAGTATGAGA, which resulted in a 1323 bp deletion beginning at Chromosome 18 position 32,079,445 bp and ending after 32,080,767 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000412450 (exon 3) and 416 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 50 and early truncation 29 amino acids later. |
