| Primary Identifier | MGI:6306307 | Allele Type | Transgenic |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Tg(HBB*)L2Pai |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | This transgene contains a 183-kb fragment of the human beta-globin locus with the codons 41-42 (-TTCT) deletion mutation. The L2 line contains six copies of the transgene. RT-PCR and sequencing indicated the production of a 156-bp product confirming the presence of the 4-bp deletion. In addition, a 118-bp PCR product was also detected and sequencing of this aberrant splicing product indicates a deletion of 38-bp of exon 1 (codons 18-30). |
