| Primary Identifier | MGI:6278139 | Allele Type | Targeted |
| Attribute String | Null/knockout, Recombinase, Reporter | Gene | Kiss1 |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The targeting construct contains a Cre:GFP fusion carrying Myc 3' UTR pA, and an FRT-SvNeo-FRT cassette introduced to the end of the first exon, such that the sequence between the start codon and the end of that exon are deleted. Flp-mediated recombination removed the FRT-flanked neo cassette. The construct removes the nuclear localization signal from cre, uses a non-optimal initiation codon, removes part of the N-terminal sequence of cre, adds a 3' untranslated region from the Myc gene that promotes a short mRNA half-life, and deletes part of exon 1 that includes the signal peptide. |
