| Primary Identifier | MGI:6295872 | Allele Type | Targeted |
| Attribute String | Inducible, Reporter, Transactivator | Gene | Col1a1 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Induced With | doxycycline/tetracycline | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The transgene construct was inserted using recombinase-mediated cassette exchange (RMCE). The transgene construct contains the following elements: the CAG promoter driving the expression of the reverse tetracycline-controlled transactivator, and the tet operon tetracycline responsive element driving the expression of turboGFP green fluorescent protein reporter. The GFP gene is fused with sequence for a miR30-based shRNA targeting Renilla reniformis luciferase. Only upon supplying mice carrying this allele with doxycycline (or tetracycline) will the GFP/shRNA hybrid gene be expressed. |
