Primary Identifier | MGI:6313718 | Allele Type | Targeted |
Attribute String | Conditional ready, Null/knockout, Reporter | Gene | Cela2a |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The transgene construct, plasmid PRPGS00048_A_H09 (KOMP project), was electroporated into ES cells. Only clone 2B9 showed proper recombination and was chosen to establish the mouse line. The allele comprises the following elements: an FRT site, a gene trap cassette (mouse En2 splice acceptor, IRES, LacZ gene, SV40 poly(A) signal sequences), a loxP site, a neomycin resistance gene cassette, a second loxP site and a aecond FRT site in intron 2, and a third loxP site in intron 5. |