| Primary Identifier | MGI:6314206 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Dnajb14 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TCTTCTGGAGAGACTAAACA and ATATAGCACAGTTTAATAGA, which resulted in a 396 bp deletion beginning at Chromosome 3 position 137,885,162 bp and ending after 137,885,557 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001225953 (exon 2) and 224 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause an early truncation after amino acids 45. There is an 8 bp insertion (TTCATAGC) at the deletion site and a single bp insertion (T) 11 bp before the deletion site. |
