| Primary Identifier | MGI:6305217 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Pcnx4 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CTATTTACCAAGATAAACTG and CCACAGTTACACTCAGCCAC, which resulted in a 198 bp deletion beginning at Chromosome 12 position 72,556,521 bp and ending after 72,556,718 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000437603 (exon 5) and 97 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 464 and early truncation 32 amino acids later. |
