| Primary Identifier | MGI:6336088 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cfap144 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 1 and approximately 1.5 kb of sequence upstream of exon 1 (promotor region) was flanked by loxP sites. A FRT-flanked puromycin resistance gene was inserted into intron 1 and removed via Flp-mediated recombination. Cre-mediated recombination removed the floxed region deleting the promotor region and transcriptional and translational start sites. Western blot analysis of testis lysates confirmed absence of protein in homozygotes. |
