| Primary Identifier | MGI:6316171 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Chst14 |
| Strain of Origin | C57BL/6NCrl | Is Recombinase | false |
| Is Wild Type | false | Project Collection | IMPC |
| molecularNote | This allele from project TCPR259 was generated at The Centre for Phenogenomics.It was targeted by CRISPR/Cas9 nickase-mediated single-strand DNA nicks to induce double-strand breaks repaired by NHEJ causing frame-shift mutation(s) in exon 1 of Chst14. The two guides were targeted to the forward strand of Chr 2:118926702:118926721 and the reverse strand of Chr 2:118926686:118926705 (GRCm38). |
